ISO с низкой стоимостью потребления Экономичная венчурная машина

VC Project Introduction

Manufacturing vitamin C involves four key steps: fermentation, extraction, conversion, and refinement.

1. Fermentation section

Connecting the purified strain ramp into the empty sterile ramp in the strain room and culture to the endpoint constitute the bulk of the task. They were then injected into a triangle flask filled with sterile media, where it was cultivated till maturity. Kirschner flask media was used for cultivation all the way through. The sterile medium in the small fermentation tank was then attached to the seed liquid prepared in a Kirschner flask. After proper cultivation, the hygienic was canned and chilled, and a sterility check was performed. Following qualification, the seed was given to fermentation.

Fermentation starts with a four-stage expansion culture. The expansion of the seed solution occurs during the first, second, and third stages. Fermentation occurs in four sets to produce sorbic sugar. The first step seed solution was connected to the first-level sterile medium for culture to the endpoint, followed by connections to the second-level sterile medium and the third-level sterile medium for culture to the endpoint, and finally, links to fermentation and the fourth-level sterile medium. After the fermentation tank's first stage achieves its conclusion, regulate the temperature, tank pressure, and sterile airflow while the fermentation process is underway, and prepare sugar.

Four-stage expansion culture is the second stage of fermentation. The expansion of the seed solution occurs during the first, second, and third stages. Fermentation occurs in four stages to produce sodium cologne acid. In the second step, the seed solution was connected to the first-level sterile medium for culture to the endpoint, then to the second-level sterile medium, the third-level sterile medium, and the fourth-level sterile medium for culture to the endpoint, and finally connected to fermentation. The temperature, tank pressure, and sterile air flow were all controlled during the fermentation process, and the feed liquid's pH was adjusted to be slightly acidic. After that, the material was ready to be discharged until the fermentation reached its endpoint.

2. Extraction section

The fermenting liquid is added to the refinery feeding tank and then transferred via an oscillating sieve into the circulating tank. Discharge the dialysate into the clear liquid tank after starting the membrane filtration system for filtration. When the produced concentration is kept under 10mg/ml, it is treated for environmental protection. After multi-stage concentration and desalination using resin, the dialysate goes into the crystallizer to chill and crystallize. Control the crystallization temperature at 5.0 and separate the solid from the liquid using a centrifuge to obtain wet gluonic acid. The mother liquid that has been dissolved is concentrated twice, cooled, and crystallized at 5–10°C. Moist cologonic acid that has been recovered and separated enters the conversion portion.

3. Conversion section

Quantitatively determined wet gluonic acid or recovered gluconic acid is added after the concentrated H2SO4 is introduced gently with open stirring in the ester conversion tank. The reaction is then heated to the endpoint. The conversion reaction is carried out after the feed material has cooled, and the reaction's control feed material has a modest alkaline pH. After the response has achieved its endpoint, the substance is cooled through a cooling tank to less than ten °C in preparation for solid-liquid separation. After the sodium vitamin C dissolves, there is an ion exchange process to desalt and decolorize the mixture, followed by many concentrations before entering the crystallization tank for cooling. The mother liquid of methanol is transferred for distillation and purification before use. Crude vitamin C was obtained by centrifuging the crystallized liquid after cooling it with cold brine to below 5.0°C. The raw vitamin C can be refined or coarsely dissolved in clean water.

By concentration, crystallization, cooling, and separation, the mother liquor of crude vitamin C is divided to obtain the first recovered vitamin C, which is subsequently dissolved into the desalination apparatus for use. After cooling and neutralizing the secondary mother liquor, alcohol precipitated and separated the solid cologne acid sodium for desalting. In the first mother liquor concentration system, the separated third mother liquor was desalted, decolorized, and dealcoholized.

4. Refinery section

Add water to boil up and dissolve crude vitamin C and carbon to decolorize, following the technical requirements. It goes through multiple stages of filtering before entering the crystallization tank. Use lead crystals and carefully manage to cool to achieve control over crystal size dispersion. Once the feeding of the crystallization tank is complete, control the cooling speed to let the material cool to the crystallization point before adding the proper quantity of crystal seed. Add the antifreeze solvent after lowering the temperature till it is 0°C below to discharge. Use a centrifuge to separate the solid from the liquid and to drain the mother liquid's washing liquid. The dryer is used to dry the wet vitamin C. The drying temperature is kept under 65°C. 3 hours for drying. The substance is cooled and released after drying. The dryer uses pressure reduction during the drying process to safeguard the material. The material goes through screening and gold examination after drying. When the inspection is successful, storage is followed by weighing and packaging.

The dealcoholized mother liquor is processed after being desalted in the conversion step. The distillation column corrects the separated crude methanol, which is then employed in the refinery portion.